Changsheng, Chai, He Hung, Ning Li, Dechen, Jia, Sheng Yang,Weihong Jiang, Yang Gu
Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China
Metabolic regulation and engineering of Clostridia for substrate utilization and chemical synthesis
The use of fossil fuels is no longer tenable and their use is damaging the environment through pollution and global warming. Alternatively, environmentally friendly sources of chemicals and fuels are required. One alternative is to directly capture carbon before incorporation into lignocellulosic biomass. Acetogenic bacteria, typified by Clostridium ljungdahlii, are able to capture carbon (CO or CO2) through the Wood-Ljungdahl pathway, allowing them to grow on a spectrum of waste gases from industry (e.g., steel manufacture and oil refining, coal and natural gas). They can also consume ‘synthesis gas’ (mixtures of CO, CO2 and H2) made from the gasification of renewable resources, such as biomass in the form of domestic or agricultural wastes. Here, we report the development of a highly efficient CRISPR/Cas9 system and transposon mutagenesis system for rapid genome editing of C. ljungdahlii, as well as metabolic design and engineering of this anaerobe to produce a variety of important chemical and biofuels using C1 gases.
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